A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Mohammadniaei, M, Zhang, M, Ashley, J, Christensen, UB, Friis-Hansen, LJ, Gregersen, R, Lisby, JG, Benfield, TL, Nielsen, FE, Rasmussen, JH, Pedersen, EB, Olinger, ACR, Kolding, LT, Naseri, M, Zheng, T, Wang, W, Gorodkin, J and Sun, Y (2021) A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications, 12 (1). ISSN 2041-1723

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Abstract

The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

Item Type:	Article
Additional Information:	Embargo requested: Not known
Uncontrolled Keywords:	Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; NUCLEIC-ACIDS; COVID-19
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Nature Publishing Group
Related URLs:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000688006200010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=7f77ca1697db64ccb27a8011c7ced90d
Date of acceptance:	9 August 2021
Date of first compliant Open Access:	4 April 2022
Date Deposited:	04 Apr 2022 09:39
Last Modified:	04 Apr 2022 09:45
DOI or ID number:	10.1038/s41467-021-25387-9
URI:	https://researchonline.ljmu.ac.uk/id/eprint/16583

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