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Mohammadniaei, M, Zhang, M, Ashley, J, Christensen, UB, Friis-Hansen, LJ, Gregersen, R, Lisby, JG, Benfield, TL, Nielsen, FE, Rasmussen, JH, Pedersen, EB, Olinger, ACR, Kolding, LT, Naseri, M, Zheng, T, Wang, W, Gorodkin, J and Sun, Y (2021) A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications, 12 (1). ISSN 2041-1723
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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
| Item Type: | Article |
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| Additional Information: | Embargo requested: Not known |
| Uncontrolled Keywords: | Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; NUCLEIC-ACIDS; COVID-19 |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Nature Publishing Group |
| Related URLs: | |
| Date Deposited: | 04 Apr 2022 09:39 |
| Last Modified: | 04 Apr 2022 09:45 |
| DOI or ID number: | 10.1038/s41467-021-25387-9 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/16583 |
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