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Vallabh, NA, Armstrong, J, Czanner, G, McDonagh, B, Choudhary, A, Criddle, DN and Willoughby, CE (2022) Evidence of impaired mitochondrial cellular bioenergetics in ocular fibroblasts derived from glaucoma patients. Free Radical Biology and Medicine, 189. pp. 102-110. ISSN 0891-5849
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Evidence of impaired mitochondrial cellular bioenergetics in ocular fibroblasts derived from glaucoma patients.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (834kB) | Preview |
Abstract
Glaucoma is a progressive optic neuropathy characterized by the neurodegeneration of the retinal ganglion cells (RGCs) resulting in irreversible visual impairment and eventual blindness. RGCs are extremely susceptible to mitochondrial compromise due to their marked bioenergetic requirements and morphology. There is increasing interest in therapies targeting mitochondrial health as a method of preventing visual loss in managing glaucoma. The bioenergetic profile of Tenon's ocular fibroblasts from glaucoma patients and controls was investigated using the Seahorse XF24 analyser. Impaired mitochondrial cellular bioenergetics was detected in glaucomatous ocular fibroblasts including basal respiration, maximal respiration and spare capacity. Spare respiratory capacity levels reflect mitochondrial bio-energetic adaptability in response to pathophysiological stress. Basal oxidative stress was elevated in glaucomatous Tenon's ocular fibroblasts and hydrogen peroxide (H2O2) induced reactive oxygen species (ROS) simulated the glaucomatous condition in normal Tenon's ocular fibroblasts. This work supports the role of therapeutic interventions to target oxidative stress or provide mitochondrial energetic support in glaucoma.
| Item Type: | Article |
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| Uncontrolled Keywords: | Mitochondria; Fibroblasts; Humans; Glaucoma; Hydrogen Peroxide; Energy Metabolism; Bioenergetics; Glaucoma; Mitochondria; Oxidative stress; Seahorse XF analyser; Tenon's fibroblast; Energy Metabolism; Fibroblasts; Glaucoma; Humans; Hydrogen Peroxide; Mitochondria; Biochemistry & Molecular Biology; 0304 Medicinal and Biomolecular Chemistry; 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics |
| Subjects: | R Medicine > R Medicine (General) T Technology > T Technology (General) |
| Divisions: | Computer Science & Mathematics |
| Publisher: | Elsevier BV |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 16 Sep 2022 09:20 |
| Last Modified: | 16 Sep 2022 09:30 |
| DOI or ID number: | 10.1016/j.freeradbiomed.2022.07.009 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/17589 |
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