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Wilson, M, Birkett, JW, Khan, I, Abbas, I, Tang, L and Assi, S (2023) The Detection of Biomarkers and Cocaine in Fingernails Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. Spectroscopy, 38 (S8). pp. 37-44. ISSN 0887-6703
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The Detection of Biomarkers and Cocaine in Fingernails Using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy .pdf - Accepted Version Download (805kB) | Preview |
Abstract
Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) provides portable and rapid analysis of biomarkers and drugs within fingernails. Nails offer a suitable alternative to traditional biological matrices and provide advantages such as non-invasive collection and small sample sizes. This work utilised ATR-FTIR for the detection of biomarkers and cocaine within fingernails. Fingernails were analyzed initially ‘as received’ to identify biomarkers such as carbohydrates, lipids, and proteins over the wavenumber range 650-4000 cm-1. Spectra were collected for fingernails before and after spiking with cocaine hydrochloride. Measurements were taken at one week and up to six weeks. The results showed that FTIR spectra of fingernails had bands corresponding to carbohydrates at 926 and 1045 cm-1, lipid bands at 2958, 2921 and 2851 cm-1, and protein bands at 1644 and 1536 cm-1. Analysis of cocaine-spiked fingernails demonstrated cocaine bands at 978 cm-1 (monosubstituted out-of-plane bending vibration), 1010 cm-1 (CO stretching), 1027 cm-1 (monosubstituted benzene ring stretching), 1055 cm-1 (CO and CN stretching), 1071 cm-1 (monosubstituted benzene ring stretching), and 1105 cm-1 (CO stretching). Principal component analysis (PCA) revealed discrete clusters within the PC scores of cocaine-spiked versus un-spiked fingernails. Findings showed that ATR-FTIR spectroscopy could characterize fingernails based on intrinsic components and identify the presence or absence of cocaine within them.
| Item Type: | Article |
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| Uncontrolled Keywords: | spectroscopy; infrared; infrared spectroscopy; cocaine; drug detection; fingernails |
| Subjects: | R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | MJH Lifesciences |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 24 Apr 2023 11:08 |
| Last Modified: | 20 Sep 2023 15:15 |
| DOI or ID number: | 10.56530/spectroscopy.oe1567k3 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/18924 |
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