Analysis of non-conjugated steroids in water using paper spray mass spectrometry

Jjunju, FPM, Damon, DE, Romero-Perez, D, Young, IS, Ward, RJ ORCID: 0000-0002-9850-5191, Marshall, A, Maher, S and Badu-Tawiah, AK (2020) Analysis of non-conjugated steroids in water using paper spray mass spectrometry. Scientific Reports, 10 (1). ISSN 2045-2322

Preview

Text Analysis of non-conjugated steroids in water using paper spray mass spectrometry.pdf - Published Version Available under License Creative Commons Attribution. Download (4MB) | Preview

Abstract

A novel strategy for the direct analysis of non-conjugated steroids in water using paper spray mass spectrometry (PS-MS) has been developed. PS-MS was used in the identification and quantification of non-conjugated (free) steroids in fish tank water samples. Data shown herein indicates that individual amounts of free steroids can be detected in aqua as low as; 0.17 ng/µL, 0.039 ng/µL, 0.43 ng/µL, 0.0076 ng/µL for aldosterone, corticosterone, cortisol, and β-estrone, respectively, and with an average relative standard deviation of ca. < 10% in the positive ion mode using PS-MS/MS. Direct detection of free steroids in a raw water mixture, from aquaculture, without prior sample preparation is demonstrated. The presence of free steroids released in fish water samples was confirmed via tandem mass spectrometry using collision-induced dissociation. This approach shows promise for rapid and direct water quality monitoring to provide a holistic assessment of non-conjugated steroids in aqua.

Item Type:	Article
Subjects:	Q Science > QA Mathematics > QA75 Electronic computers. Computer science R Medicine > R Medicine (General)
Divisions:	Computer Science and Mathematics
Publisher:	Nature Publishing Group
Date of acceptance:	1 June 2020
Date of first compliant Open Access:	5 June 2023
Date Deposited:	05 Jun 2023 11:06
Last Modified:	04 Jul 2025 18:45
DOI or ID number:	10.1038/s41598-020-67484-7
URI:	https://researchonline.ljmu.ac.uk/id/eprint/19617

View Item