Prasetyo, AP, Cusa, M, Murray, JM, Agung, F, Muttaqin, E, Mariani, S and McDevitt, AD (2023) Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots. iScience, 26 (7). ISSN 2589-0042

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Abstract

Trade restrictions for endangered elasmobranch species exist to disincentivise their exploitation and curb their declines. However, trade monitoring is challenging due to product variety and the complexity of import/export routes. We investigate the use of a portable, universal, DNA-based tool which would greatly facilitate in-situ monitoring. We collected shark and ray samples across the Island of Java, Indonesia, and selected 28 commonly encountered species (including 22 CITES-listed species) to test a recently developed real-time PCR single-assay originally developed for screening bony fish. In the absence of a bespoke elasmobranch identification online platform in the original FASTFISH-ID model, we employed a deep learning algorithm to recognize species based on DNA melt-curve signatures. By combining visual and machine learning assignment methods, we distinguished 25/28 species, 20 of which were CITES-listed. With further refinement, this method can improve monitoring of the elasmobranch trade worldwide, without a lab or species-specific assays.

Item Type:	Article
Subjects:	Q Science > QL Zoology
Divisions:	Biological & Environmental Sciences (from Sep 19)
Publisher:	Cell Press
SWORD Depositor:	A Symplectic
Date Deposited:	09 Jun 2023 10:43
Last Modified:	27 Jun 2023 10:15
DOI or ID number:	10.1016/j.isci.2023.107065
URI:	https://researchonline.ljmu.ac.uk/id/eprint/19722

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