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Bidula, S, Sexton, DW and Schelenz, S (2015) Serum opsonin ficolin-A enhances host-fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge. Medical Microbiology Immunology. ISSN 1432-1831
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Abstract
Invasive aspergillosis is a devastating invasive fungal disease associated with a high mortality rate in the immunocompromised, such as leukaemia patients, transplant patients and those with HIV/AIDS. The rodent serum orthologue of human L-ficolin, ficolin-A, can bind to and opsonize Aspergillus fumigatus, the pathogen that causes invasive aspergillosis, and may participate in fungal defence. Using human monocyte-derived macrophages and neutrophils isolated from healthy donors, we investigated conidial association and fungal viability by flow cytometry and microscopy. Additionally, cytokine production was measured via cytometric bead arrays. Ficolin-A opsonization was observed to significantly enhance association of conidia, while also inhibiting hyphal growth and contributing to increased fungal killing following incubation with monocyte-derived macrophages and neutrophils. Additionally, ficolin-A opsonization was capable of manifesting a decrease in IL-8, IL-1β, IL-6, IL-10 and TNF-α production from MDM and IL-1β, IL-6 and TNF-α from neutrophils 24 h post-infection. In conclusion, rodent ficolin-A is functionally comparable to human L-ficolin and is capable of modulating the innate immune response to A. fumigatus, down-regulating cytokine production and could play an important role in airway immunity.
| Item Type: | Article |
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| Additional Information: | The final publication is available at Springer via http://dx.doi.org/10.1007/s00430-015-0435-9 |
| Uncontrolled Keywords: | 1107 Immunology, 1108 Medical Microbiology |
| Subjects: | Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology |
| Divisions: | Pharmacy and Biomolecular Sciences |
| Publisher: | Springer Verlag |
| Related URLs: | |
| Date Deposited: | 05 Nov 2015 10:37 |
| Last Modified: | 04 Sep 2021 13:58 |
| DOI or ID number: | 10.1007/s00430-015-0435-9 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/2011 |
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Serum opsonin ficolin-A enhances host-fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge. (deposited 14 Sep 2015 11:48)
- Serum opsonin ficolin-A enhances host-fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge. (deposited 05 Nov 2015 10:37) [Currently Displayed]
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