Cheong, MS, Nahar, L, Sarker, SD, Cao, H, Cheang, WS and Xiao, JB (2024) Stability of Piceatannol in Dulbecco's Modified Eagle's Medium by In Situ UPLC-MS/MS Analysis. Natural Product Communications, 19 (1). pp. 1-11. ISSN 1934-578X

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Abstract

Piceatannol is a stilbenoid, which has shown bioactivities in various cell culture models. However, its stability in cell culture medium is not clear. Here, UPLC-MS/MS was applied in situ to analyze the degradation products of piceatannol in Dulbecco's Modified Eagle's Medium (DMEM) and cell culture to investigate the compound's stability in DMEM. During the incubation with cell culture medium (at 4 and 37 °C), several piceatannol derivatives, such as an oxidation product ( m/z 243.06), a reduction product ( m/z 247.09), dimers ( m/z 485.12 and 487.14) and trimers ( m/z 727.18) were detected, which demonstrated the instability of piceatannol in cell culture conditions. To confirm if the new products during the incubation were generated due to the instability of piceatannol, ascorbic acid was added. The presence of ascorbic acid could significantly slow the degradation rate of piceatannol and the generation of piceatannol derivatives, which proved that the new products were generated by the degradation of piceatannol and indicated that the instability of piceatannol might be related to its antioxidant activity.

Item Type:	Article
Uncontrolled Keywords:	0305 Organic Chemistry; 0602 Ecology; 0607 Plant Biology
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	SAGE Publications
SWORD Depositor:	A Symplectic
Date Deposited:	29 Jan 2024 12:15
Last Modified:	29 Jan 2024 12:15
DOI or ID number:	10.1177/1934578X221111036
URI:	https://researchonline.ljmu.ac.uk/id/eprint/22459

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