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Seenappanahalli Nanjundaiah, Y, Wright, DA, Baydoun, AR, O'Hare, WT, Ali, Z, Khaled, Z and Sarker, MH (2016) Lactobacillus rhamnosus GG conditioned media modulates acute reactive oxygen species and nitric oxide in J774 murine macrophages. Biochemistry and Biophysics Reports, 6. pp. 68-75. ISSN 2405-5808
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iLactobacillus rhamnosusi GG conditioned media modulates acute reactive oxygen species and nitric oxide in J774 murine macro.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (690kB) | Preview |
Abstract
Phagocytes such as macrophages are capable of detecting and killing pathogenic bacteria by producing reactive oxygen and nitrogen species. Formation of free radicals in macrophages may be regulated by probiotics or by factors released by probiotics but yet to be identified. Thus, studies were carried out to determine whether cell-free conditioned medium obtained from cultures of Lactobacillus rhamnosus GG (LGG-CM) regulate production of reactive oxygen species (ROS) and/or nitric oxide (NO) in macrophages. J774 macrophages in culture were loaded with either H2DCFDA for monitoring ROS or with DAFFM-DA for NO detection. Free radical production was measured on a fluorescence microplate reader and changes were analysed by Cumulative sum (CuSum) calculations. Low concentration of LGG-CM (10% LGG-CM) or LPS did not cause any significant change in basal levels of ROS or NO production. In contrast, high concentration of LGG-CM (75% and 100%) significantly enhanced ROS generation but also significantly reduced NO level. These findings are novel and suggest for the first time that probiotics may release factors in culture which enhance ROS production and may additionally reduce deleterious effects associated with excessive nitrogen species by suppressing NO level. These events may account, in part, for the beneficial bactericidal and anti-inflammatory actions ascribed to probiotics and may be of clinical relevance.
| Item Type: | Article |
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| Uncontrolled Keywords: | CuSum; In vitro; Nitric oxide; Probiotic bacteria; ROS; 0601 Biochemistry and Cell Biology |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Elsevier |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 14 Feb 2024 16:07 |
| Last Modified: | 14 Feb 2024 16:07 |
| DOI or ID number: | 10.1016/j.bbrep.2016.03.003 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/22627 |
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