Hudson, N, Powner, MB, Sarker, MH, Burgoyne, T, Campbell, M, Ockrim, ZK, Martinelli, R, Futter, CE, Grant, MB, Fraser, PA, Shima, DT, Greenwood, J and Turowski, P (2014) Differential apicobasal VEGF signaling at vascular blood-neural barriers. Developmental Cell, 30 (5). pp. 541-552. ISSN 1534-5807

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Abstract

The vascular endothelium operates in a highly polarized environment, but to date there has been littleexploration of apicobasal polarization of its signaling. We show that VEGF-A, histamine, IGFBP3, and LPA trigger unequal endothelial responses when acting from the circulation or the parenchymal side at blood-neural barriers. For VEGF-A, highly polarized receptor distribution contributed to distinct signaling patterns: VEGFR2, which was found to be predominantly abluminal, mediated increased permeability via p38; in contrast, luminal VEGFR1 led to Akt activation and facilitated cytoprotection. Importantly, such differential apicobasal signaling and VEGFR distribution were found in the microvasculature of brain and retina but not lung, indicating that endothelial cells at blood-neural barriers possess specialized signaling compartments that assign different functions depending on whether an agonist is tissue or blood borne.

Item Type:	Article
Uncontrolled Keywords:	Blood-Brain Barrier; Endothelium, Vascular; Microcirculation; Neurons; Animals; Mice, Inbred C57BL; Rats, Inbred Lew; Mice; Rats; Rats, Wistar; p38 Mitogen-Activated Protein Kinases; Vascular Endothelial Growth Factor Receptor-1; Vascular Endothelial Growth Factor Receptor-2; Vascular Endothelial Growth Factor A; Signal Transduction; Permeability; Male; Animals; Blood-Brain Barrier; Endothelium, Vascular; Male; Mice; Mice, Inbred C57BL; Microcirculation; Neurons; Permeability; Rats; Rats, Inbred Lew; Rats, Wistar; Signal Transduction; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-1; Vascular Endothelial Growth Factor Receptor-2; p38 Mitogen-Activated Protein Kinases; 06 Biological Sciences; 11 Medical and Health Sciences; Developmental Biology
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Cell Press
SWORD Depositor:	A Symplectic
Date Deposited:	28 Feb 2024 12:26
Last Modified:	28 Feb 2024 12:26
DOI or ID number:	10.1016/j.devcel.2014.06.027
URI:	https://researchonline.ljmu.ac.uk/id/eprint/22706

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