Ahmed, KA-A, Jabbar, AAJ, Galali, Y, Al-Qaaneh, AM, Akçakavak, G, Salehen, NA, Hassan, RR, Mothana, RA, Abdulla, MA, Fantoukh, OI, Hasson, S and Hawwal, MF (2024) Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms. Skin Research & Technology, 30 (5). ISSN 0909-752X

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Abstract

Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up-regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF-β1 (angiogenetic factor) was significantly more expressed in CCS-treated rats in comparison to normal saline-treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS-treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up-regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.

Item Type:	Article
Uncontrolled Keywords:	Skin; Animals; Rats; Rats, Sprague-Dawley; Cuminum; Seeds; Plant Extracts; Wound Healing; Male; Transforming Growth Factor beta1; Cuminum cyminum L.; antioxidants; histology; immunohistochemistry; wound; Animals; Wound Healing; Rats, Sprague-Dawley; Seeds; Rats; Plant Extracts; Cuminum; Male; Skin; Transforming Growth Factor beta1; 1103 Clinical Sciences
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Wiley
SWORD Depositor:	A Symplectic
Date Deposited:	22 May 2024 09:40
Last Modified:	22 May 2024 09:45
DOI or ID number:	10.1111/srt.13727
URI:	https://researchonline.ljmu.ac.uk/id/eprint/23339

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