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Millichap, LE, Turton, N, Damiani, E, Marcheggiani, F, Orlando, P, Silvestri, S, Tiano, L and Hargreaves, IP (2024) The Effect of Neuronal CoQ10 Deficiency and Mitochondrial Dysfunction on a Rotenone-Induced Neuronal Cell Model of Parkinson’s Disease. International Journal of Molecular Sciences, 25 (12). ISSN 1661-6596
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The Effect of Neuronal CoQ10 Deficiency and Mitochondrial Dysfunction on a Rotenone-Induced Neuronal Cell Model of Parkinson’s Disease.pdf - Published Version Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder currently affecting the ageing population. Although the aetiology of PD has yet to be fully elucidated, environmental factors such as exposure to the naturally occurring neurotoxin rotenone has been associated with an increased risk of developing PD. Rotenone inhibits mitochondrial respiratory chain (MRC) complex I activity as well as induces dopaminergic neuronal death. The aim of the present study was to investigate the underlying mechanisms of rotenone-induced mitochondrial dysfunction and oxidative stress in an in vitro SH-SY5Y neuronal cell model of PD and to assess the ability of pre-treatment with Coenzyme Q10 (CoQ10) to ameliorate oxidative stress in this model. Spectrophotometric determination of the mitochondrial enzyme activities and fluorescence probe studies of reactive oxygen species (ROS) production was assessed. Significant inhibition of MRC complex I and II-III activities was observed, together with a significant loss of neuronal viability, CoQ10 status, and ATP synthesis. Additionally, significant increases were observed in intracellular and mitochondrial ROS production. Remarkably, CoQ10 supplementation was found to reduce ROS formation. These results have indicated mitochondrial dysfunction and increased oxidative stress in a rotenone-induced neuronal cell model of PD that was ameliorated by CoQ10 supplementation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 0399 Other Chemical Sciences; 0604 Genetics; 0699 Other Biological Sciences; Chemical Physics |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | MDPI |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 18 Jun 2024 11:44 |
| Last Modified: | 18 Jun 2024 11:45 |
| DOI or ID number: | 10.3390/ijms25126622 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/23603 |
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