Arabnozari, H, Shaki, F, Najjari, A, Sharifianjazi, F, Sarker, SD, Habibi, E and Nahar, L (2024) The effect of Polygonum hyrcanicum Rech. f. hydroalcoholic extract on oxidative stress and nephropathy in alloxan-induced diabetic mice. Scientific Reports, 14 (1).

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Abstract

Diabetic nephropathy, characterized by inflammation and oxidative stress, poses a management challenge. This study investigates the effect of Polygonum hyrcanicum extract on diabetic nephropathy in alloxan-induced diabetic mice. In this experimental animal study, the P. hyrcanicum extract was prepared using continuous macerations. Thirty male Albino mice, divided into five groups, were induced with alloxan-induced diabetes. They received intraperitoneal injections of the plant extract (100 and 200 mg/kg) and metformin (300 mg/kg) for four weeks. Kidney and blood samples were collected to assess protein carbonyl, glutathione, lipid peroxidation, TNF-α and IL-6 levels. The amount of total flavonoid and phenolic content in the hydroalcoholic extract of P. hyrcanicum were 7.5 ± 0.3 mg of quercetin and 88.2 ± 1.3 mg gallic acid per gram of extract respectively. The antioxidant activity level of the hydroalcoholic extract was determined to be 1.78 ± 0.51 mM equivalent per gram of extract. Alloxan administration resulted in a significant reduction in glutathione levels and a significant increase in protein carbonyl, lipid peroxidation, TNF-α, and IL-6 levels. Hydroalcoholic extract of P. hyrcanicum effectively reduced oxidative stress markers and inflammatory cytokines (TNF-α, IL-6), indicating its potential in mitigating diabetic nephropathy. However, no significant difference in efficacy was observed between the 100 mg/kg and 200 mg/kg doses in terms of reducing these toxicities.

Item Type:	Article
Uncontrolled Keywords:	Kidney; Animals; Mice; Polygonum; Diabetic Nephropathies; Diabetes Mellitus, Experimental; Alloxan; Tumor Necrosis Factor-alpha; Glutathione; Plant Extracts; Interleukin-6; Antioxidants; Lipid Peroxidation; Oxidative Stress; Male; Polygonum hyrcanicum; Alloxan; Diabetes; Nephropathy; Oxidative stress; Animals; Oxidative Stress; Plant Extracts; Diabetes Mellitus, Experimental; Diabetic Nephropathies; Mice; Male; Antioxidants; Polygonum; Alloxan; Lipid Peroxidation; Tumor Necrosis Factor-alpha; Glutathione; Kidney; Interleukin-6
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Nature Research
SWORD Depositor:	A Symplectic
Date Deposited:	30 Aug 2024 13:28
Last Modified:	30 Aug 2024 13:30
DOI or ID number:	10.1038/s41598-024-69220-x
URI:	https://researchonline.ljmu.ac.uk/id/eprint/24048

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