Gillham, SH, Cole, PL, Viggars, MR, Nolan, AH, Close, GL and Owens, DJ (2024) Comparative transcriptomics of broad-spectrum and synthetic cannabidiol treated C2C12 skeletal myotubes. Physiological reports, 12 (18).

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Abstract

Cannabidiol (CBD) is widely used in sports for recovery, pain management, and sleep improvement, yet its effects on muscle are not well understood. This study aimed to determine the transcriptional response of murine skeletal muscle myotubes to broad-spectrum CBD and synthetic CBD (sCBD). Differentiated C2C12 myotubes were treated with 10 μM CBD, sCBD, or vehicle control (DMSO) for 24 h before RNA extraction. Poly-A tail-enriched mRNA libraries were constructed and sequenced using 2 × 50 bp paired-end sequencing. CBD and sCBD treatment induced 4489 and 1979 differentially expressed genes (DEGs; p < 0.001, FDR step-up <0.05), respectively, with common upregulation of 857 genes and common downregulation of 648 genes. Common upregulated DEGs were associated with "response to unfolded protein," "cell redox homeostasis," "endoplasmic reticulum stress," "oxidative stress," and "cellular response to hypoxia." Common downregulated DEGs were linked to "sarcomere organization," "skeletal muscle tissue development," "regulation of muscle contraction," and "muscle contraction." CBD treatment induced unique DEGs compared to sCBD. The data indicate CBD may induce mild cellular stress, activating pathways associated with altered redox balance, unfolded protein response, and endoplasmic reticulum stress. We hypothesize that CBD interacts with muscle and may elicit a "mitohormetic" effect that warrants further investigation.

Item Type:	Article
Uncontrolled Keywords:	Cell Line; Animals; Mice; Cannabidiol; Muscle Fibers, Skeletal; Transcriptome; cannabidiol; skeletal muscle; transcriptome; Cannabidiol; Animals; Mice; Muscle Fibers, Skeletal; Transcriptome; Cell Line; 0606 Physiology; 1103 Clinical Sciences; 1116 Medical Physiology
Subjects:	R Medicine > RC Internal medicine > RC1200 Sports Medicine R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Sport and Exercise Sciences
Publisher:	Wiley
SWORD Depositor:	A Symplectic
Date Deposited:	01 Oct 2024 08:52
Last Modified:	01 Oct 2024 09:00
DOI or ID number:	10.14814/phy2.70059
URI:	https://researchonline.ljmu.ac.uk/id/eprint/24301

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