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Cunnington, AV, Shum, P, Wilding, CS and Mariani, S (2024) Exploring intertidal sea anemones Actinia equina as natural eDNA samplers for coastal biodiversity assessment. Marine Ecology Progress Series, 743. pp. 159-165. ISSN 0171-8630
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Exploring intertidal sea anemones Actinia equina as natural eDNA samplers for coastal biodiversity assessment.pdf - Accepted Version Available under License Creative Commons Attribution. Download (459kB) | Preview |
Abstract
Biodiversity in coastal marine environments is under unprecedented threat from anthropogenic impacts, which highlights a need for cost-effective and expedient survey methods. The analysis of environmental DNA (eDNA)—typically obtained through artificially filtered water samples—can paint a detailed picture of fish diversity in marine coastal environments. More recently, the analysis of natural sampler DNA (nsDNA, based on filter-feeding invertebrates that naturally trap eDNA in their tissues) has emerged as a potential alternative to water filtering. In this study, we investigate the widely distributed beadlet anemone Actinia equina as a potential natural eDNA sampler. Anemone samples were collected from 2 coastal locations in the UK: Rhosneigr (Anglesey, North Wales) and New Brighton (Wirral, north-western England). Sampling took place over 2 different months, and samples were compared to concomitantly sampled water. DNA metabarcoding via 12S Tele02 fish-specific primers revealed successful detection of a range of fish and other vertebrate species. We observed differences in species detected between conventional eDNA and sea anemone nsDNA samples, as well as a significant difference in seasonality detected through nsDNA. Our results indicate that the beadlet anemone can be a successful natural eDNA sampler, but that its value is more likely to reside in its complementarity alongside established eDNA methods.
| Item Type: | Article |
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| Additional Information: | Cunnington AV, Shum P, Wilding CS, Mariani S (2024) Exploring intertidal sea anemones Actinia equina as natural eDNA samplers for coastal biodiversity assessment. Mar Ecol Prog Ser 743:159-165. https://doi.org/10.3354/meps14650 |
| Uncontrolled Keywords: | 0405 Oceanography; 0602 Ecology; 0608 Zoology; Marine Biology & Hydrobiology |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
| Divisions: | Biological and Environmental Sciences (from Sep 19) |
| Publisher: | Inter-Research Science Center |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 01 Oct 2024 09:30 |
| Last Modified: | 01 Oct 2024 09:30 |
| DOI or ID number: | 10.3354/meps14650 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/24306 |
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