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Mvelase, ST, Benson, SO, Omondi, RO, Kumah, RT, Fatokun, AA and Ojwach, SO (2024) Structural, DNA/BSA binding interactions and cytotoxicity studies of carboxamide (pyridyl)pyrazine palladium(II) complexes. Journal of Molecular Structure, 1322 (1). p. 140267. ISSN 0022-2860
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Mvelase et al. (2024) J Mol Structure 1322, 140267 (11 pages).pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (2MB) | Preview |
Abstract
Reactions of ligands [N2, N3-bis(pyridin-2-yl)pyrazine-2,3-dicarboxamide] (L1), [N2, N3-bis(6-methylpyridin-2- yl)pyrazine-2,3-dicarboxamide] (L2), [N2,N3-bis(4-methylpyridin-2-yl)pyrazine-2,3-dicarboxamide] (L3) and [N2, N3-bis(quinoline-8-yl)pyrazine-2,3-dicarboxamide] (L4) with [PdCl2(NCMe)2] afforded the respective palladium(II) complexes: [Pd2(L1)2Cl2] (Pd1), [Pd2(L2)2Cl2] (Pd2), [Pd2(L3)2Cl2] (Pd3) and [Pd(L4)Cl] (Pd4). Molecular structures of complexes Pd1 and Pd3 are dinuclear containing two bridging bidentate ligand units. The interactions of the palladium complexes (Pd1-Pd4) with calf thymus DNA (CT-DNA) were monitored using UV–Vis and fluorescence spectroscopy and revealed intercalative binding modes, with intrinsic binding constants (Kb) in the order of 106 M1. Bovine serum albumin (BSA) interaction was evaluated using fluorescence techniques and displayed a static quenching mechanism. The cytotoxic effects of the complexes Pd1-Pd4 were examined against human breast cancer cell lines MCF-7 and MDA-MB-231, and human transformed lung cell line MRC5-SV2 (a model of lung cancer) and its parental normal lung fibroblast cell line MRC5. While the complexes Pd1 and Pd4 showed significant to moderate cytotoxicity against MCF-7 (IC50 of 11.2 µM and 61.5 µM, respectively), complexes Pd2 and Pd3 were inactive. All the complexes were inactive against the MDA-MB-231 cell line, and Pd2-Pd4 were inactive against the MRC5-SV2 cell line. Compounds Pd1 exhibited lower cytotoxicity against the normal cell line MRC5.
| Item Type: | Article |
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| Uncontrolled Keywords: | 0306 Physical Chemistry (incl. Structural); 0307 Theoretical and Computational Chemistry; Inorganic & Nuclear Chemistry |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Pharmacy and Biomolecular Sciences |
| Publisher: | Elsevier BV |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 16 Oct 2024 09:16 |
| Last Modified: | 16 Oct 2024 09:30 |
| DOI or ID number: | 10.1016/j.molstruc.2024.140267 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/24529 |
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