Pan, D, Xia, M, Li, C, Liu, X, Archdeacon, L, O’Reilly, AO, Yuan, G, Wang, J and Dou, W (2023) CYP4CL2 Confers Metabolic Resistance to Pyridaben in the Citrus Pest Mite Panonychus citri. Journal of Agricultural and Food Chemistry, 71 (49). pp. 19465-19474. ISSN 0021-8561

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Abstract

The citrus red mite Panonychus citri has developed strong resistance to acaricides. Cytochrome P450 monooxygenases (P450s) can detoxify pesticides and are involved in pesticide resistance in many insects. Here, a pyridaben-resistant P. citri strain showed cross-resistance to cyenopyrafen, bifenazate, fenpyroximate, and tolfenpyrad. Piperonyl butoxide, a P450 inhibitor, significantly increased the toxicity of pyridaben to resistant (Pyr_Rs) and susceptible (Pyr_Control) P. citri strains. P450 activity was significantly higher in Pyr_Rs than in Pyr_Control. Analyses of RNA-Seq data identified a P450 gene (CYP4CL2) that is potentially involved in pyridaben resistance. Consistently, it was up-regulated in two field-derived resistant populations (CQ_WZ and CQ_TN). RNA interference-mediated knockdown of CYP4CL2 significantly decreased the pyridaben resistance in P. citri. Transgenic Drosophila melanogaster expressing CYP4CL2 showed increased pyridaben resistance. Molecular docking analysis showed that pyridaben could bind to several amino acids at substrate recognition sites in CYP4CL2. These findings shed light on P450-mediated pyridaben resistance in pest mites.

Item Type:	Article
Additional Information:	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/page/policy/articlesonrequest/index.html
Uncontrolled Keywords:	pyridabencross-resistance; enzymatic characteristics; RNAi; transgenic flies; molecular model; Animals; Mites; Tetranychidae; Drosophila melanogaster; Citrus; Cytochrome P-450 Enzyme System; Acaricides; Molecular Docking Simulation; RNAi; enzymatic characteristics; molecular model; pyridaben cross-resistance; transgenic flies; Animals; Mites; Citrus; Drosophila melanogaster; Molecular Docking Simulation; Tetranychidae; Acaricides; Cytochrome P-450 Enzyme System; 03 Chemical Sciences; 07 Agricultural and Veterinary Sciences; 09 Engineering; Food Science
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology
Divisions:	Biological and Environmental Sciences (from Sep 19)
Publisher:	American Chemical Society (ACS)
SWORD Depositor:	A Symplectic
Date Deposited:	07 Nov 2024 15:10
Last Modified:	04 Dec 2024 00:50
DOI or ID number:	10.1021/acs.jafc.3c06921
URI:	https://researchonline.ljmu.ac.uk/id/eprint/24702

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