Nasim, I, Ragavendran, C, Kamaraj, C, Habes Almutairi, H, Waqas Alam, M, Manimaran, K and Nakouti, I (2024) Green synthesis of ZnO nanoparticles and biological applications as broad-spectrum bactericidal, antibiofilm effects and biocompatibility studies on Zebrafish embryo. Inorganic Chemistry Communications, 169. ISSN 1387-7003

Text Green synthesis of ZnO nanoparticles and biological applications as broad-spectrum bactericidal, antibiofilm effects and biocompatibility studies on Zebrafish embryo.pdf - Accepted Version Restricted to Repository staff only until 26 August 2025. Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (636kB)

Abstract

This work generated zinc oxide nanoparticles (ZnO NPs) using Ocimum tenuiflorum in an inexpensive and environmentally friendly manner. The generated ZnO NPs were analysed using Ultra visible spectroscopy (UV–Vis), Fourier transformed infrared spectroscopy (FTIR), Scanning electron microscopy-Energy X-ray diffraction spectroscopy (SEM-EDX), Transmission electron microscopy (TEM), and X-ray diffraction (XRD). Bio-synthesized ZnO NPs showed UV–Visible absorbance maxima at 290 nm, indicating bio reduction and stability. The DRS spectra revealed a straight band gap value of 3.24 eV. Alcohols, alkanes, phenols, and amines in FTIR may contain reduced and capped zinc ions. Crystalline ZnO nanoparticles were verified by XRD. Biosynthesized ZnO NPs were exhibited in spherical and 5–35.49 nm in size by FESEM and TEM. EDX confirmed NPs zinc elemental signatures. Antibacterial properties showed that ZnO NPs effectively inhibited gram (+ve) and gram (−ve) pathogens, including E. coli (20.0 ± 0.8 mm), E. faecalis (20.0 ± 0.5 mm), S. aureus (18.01 ± 1.2 mm), and S. mutans (17.5 ± 1.0 mm). This study shows that biosynthesized ZnO NPs inhibit biofilm growth by 0.198–1.431 O.D values (Optical density). Antibiofilm inhibition was concentration-dependent. Nanoparticles proved effective against E. coli, S. mutans, S. aureus, and E. faecalis with MICs of 15 and 25 µg/mL. Time-dependent death kinetic studies indicated that 8–12 h ZnO NPs killed all bacteria. Also, ZnO NPs had low harmful effects on zebrafish eggs at doses upto 1 mg/mL. In summary, the production of green ZnO NPs offers a sustainable and biocompatible approach that is well-suited for various biological applications.

Item Type:	Article
Uncontrolled Keywords:	0302 Inorganic Chemistry; 0399 Other Chemical Sciences; Inorganic & Nuclear Chemistry
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Elsevier
SWORD Depositor:	A Symplectic
Date Deposited:	08 Nov 2024 14:23
Last Modified:	08 Nov 2024 14:30
DOI or ID number:	10.1016/j.inoche.2024.113049
URI:	https://researchonline.ljmu.ac.uk/id/eprint/24720

View Item