Shepherd, C, Lawson-Williams, M, Holland, A, Bello, AJ, Sexton, DW and Olorunniji, FJ (2025) Conditional Split Inteins: Adaptable Tools for Programming Protein Functions. International Journal of Molecular Sciences, 26 (2). ISSN 1661-6596

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Abstract

Split inteins are biological mechanisms for the operation of the spatiotemporal control of protein activities. They function through protein trans-splicing, in which their N- and C-terminal fragments are expressed contiguously with two protein halves. The subsequent self-excision upon recognition of the complimentary fragment yields a mature, complete, and functional protein. The conditional regulation of protein splicing through environmental factors or the attachment of regulatory modules can be used to determine when and where a protein will operate, providing potential novel approaches for engineering biology applications. This review will discuss current split intein applications and the mechanistic basis for novel species classification. These considerations can provide guidance in intein and extein engineering through activation strategies, in the design of spatial arrangements, and in taking advantage of unique reaction environments. This can pave the way for the future implementation of novel split intein discoveries and the selection of appropriate intein species and aid in designing novel protein engineering strategies.

Item Type:	Article
Uncontrolled Keywords:	0399 Other Chemical Sciences; 0604 Genetics; 0699 Other Biological Sciences; Chemical Physics
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	MDPI
SWORD Depositor:	A Symplectic
Date Deposited:	14 Jan 2025 15:31
Last Modified:	14 Jan 2025 15:45
DOI or ID number:	10.3390/ijms26020586
URI:	https://researchonline.ljmu.ac.uk/id/eprint/25273

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