Ellagic Acid-Wrapped Gold Nanoparticles: Evaluation of Antimicrobial, Antioxidant, Anti-Inflammatory, and Cytotoxicity Effects of KB Cells

Soni, M, Ragavendran, C, Imath, M, Manoharadas, S and Nakouti, I ORCID: 0000-0001-9438-6300 (2025) Ellagic Acid-Wrapped Gold Nanoparticles: Evaluation of Antimicrobial, Antioxidant, Anti-Inflammatory, and Cytotoxicity Effects of KB Cells. Journal of Cluster Science, 36 (4). ISSN 1040-7278

Text Ellagic acid-Wrapped gold nanoparticles Evaluation of antimicrobial, antioxidant, anti-inflammatory, and cytotoxicity effects of KB cells.pdf - Accepted Version Access Restricted until 2 June 2026. Download (597kB)

Abstract

The green synthesis of metal nanoparticles using plant-based molecules is gaining attention for its potential in biomedical applications. The aim of present study the eco-friendly synthesis of gold nanoparticles (AuNPs) using ellagic acid, a natural antioxidant, which acted as both reducing and capping agent. The successful synthesized ellagic acid-coated AuNPs (EA-AuNPs) was indicated by a colour change to dark brown and confirmed through UV-visible spectroscopy with a peak at 587.5 nm. Transmission electron microscopy (TEM) revealed long rod-shaped nanoparticles ranging from 50 to 70 nm in size, and energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of Au, C, Cl, and N elements. Zeta potential analysis showed a stable surface charge of -17.7 mV. EA-AuNPs exhibited strong antibacterial activity, including a 37.26 ± 0.9 mm inhibition zone against Klebsiella pneumoniae at 100 µg/mL and minimum inhibitory concentration (MIC) values below 1 µg/mL against Staphylococcus aureus. The nanoparticles also demonstrated potent antioxidant activity, achieving 91% and 89% radical scavenging in DPPH and ABTS assays, respectively. Anti-inflammatory testing showed 78% membrane stabilization at 200 µg/mL. Moreover, cytotoxicity studies revealed that EA-AuNPs had an IC₅₀ of 59.58 µg/mL against oral epidermoid carcinoma (KB) cells. In-silico analysis also demonstrated a promising binding affinity of -7.3 kcal/mol with Candida albicans, suggesting notable antifungal properties. The present study highlights the multi-functional therapeutic potential of EA-AuNPs in oral health care, offering a biocompatible approach to combat microbial infections, oxidative stress, inflammation, and oral cancer. The integration of both in-vitro and in-silico results supports their future application in dental and oral medicine.

Item Type:	Article
Additional Information:	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://doi.org/10.1007/s10876-025-02852-9
Uncontrolled Keywords:	3402 Inorganic Chemistry; 3403 Macromolecular and Materials Chemistry; 34 Chemical Sciences; Nanotechnology; Infectious Diseases; Bioengineering; 0302 Inorganic Chemistry; 0399 Other Chemical Sciences; Inorganic & Nuclear Chemistry; 3402 Inorganic chemistry; 3403 Macromolecular and materials chemistry
Subjects:	R Medicine > R Medicine (General)
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Springer
Date of acceptance:	14 May 2025
Date Deposited:	04 Sep 2025 09:29
Last Modified:	04 Sep 2025 09:30
DOI or ID number:	10.1007/s10876-025-02852-9
URI:	https://researchonline.ljmu.ac.uk/id/eprint/27094

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