BBCEAS-HLPC measurements for synthetic antioxidants (TBHQ, BHA, and BHT) in deep-UV region below 300 nm

Bazina, N, Ahmed, TG, Almdaaf, M, He, J, Sarker, M ORCID: 0000-0003-4698-2161 and Islam, M (2024) BBCEAS-HLPC measurements for synthetic antioxidants (TBHQ, BHA, and BHT) in deep-UV region below 300 nm. Food Chemistry, 465 (Pt 2). ISSN 0308-8146

Preview

Text BBCEASHLPC Measurements for Synthetic Antioxidants BHQ BHA and BHT in deepUV region below 300 nm.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (881kB) | Preview

Abstract

This study investigates the integration of Broadband Cavity Enhanced Absorption Spectroscopy (BBCEAS) with High-Performance Liquid Chromatography (HPLC) to detect synthetic antioxidants; BHA, BHT, and TBHQ in the deep-UV region below 300 nm. The research addresses the need for more sensitive and cost-effective detection methods in HPLC systems, particularly for low analyte concentrations, by exploring BBCEAS as a superior alternative to conventional detectors that often fall short in sensitivity. The sensitivity of the HPLC-BBCEAS system is assessed by calculating the minimum detectable change in the absorption coefficient (αmin) and comparing with those obtained using conventional HPLC and single-wavelength HPLC-CRDS methods. The findings demonstrate that the HPLC-BBCEAS system achieves αmin values of 1.8 × 10 −5 cm −1 for BHA, 2.8 × 10 −5 cm −1 for BHT, and 1.9 × 10 −5 cm −1 for TBHQ at 280 nm, with Limits of Detection (LOD) and Quantification (LOQ) up to 30 times lower than conventional HPLC systems.

Item Type:	Article
Uncontrolled Keywords:	Food Science; BBCEAS-HPLC; Deep-UV spectroscopy; Synthetic antioxidants; Butylated Hydroxyanisole; Butylated Hydroxytoluene; Hydroquinones; Antioxidants; Chromatography, High Pressure Liquid; Spectrum Analysis; Ultraviolet Rays; Limit of Detection; BBCEAS-HPLC; Deep-UV spectroscopy; Synthetic antioxidants; Antioxidants; Chromatography, High Pressure Liquid; Hydroquinones; Butylated Hydroxyanisole; Butylated Hydroxytoluene; Limit of Detection; Spectrum Analysis; Ultraviolet Rays; 30 Agricultural, Veterinary and Food Sciences; 3006 Food Sciences; Antioxidants; Chromatography, High Pressure Liquid; Hydroquinones; Butylated Hydroxyanisole; Butylated Hydroxytoluene; Limit of Detection; Spectrum Analysis; Ultraviolet Rays; Food Science; 3006 Food sciences
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Elsevier BV
Date of acceptance:	17 November 2024
Date of first compliant Open Access:	27 February 2026
Date Deposited:	27 Feb 2026 16:18
Last Modified:	27 Feb 2026 16:18
DOI or ID number:	10.1016/j.foodchem.2024.142150
URI:	https://researchonline.ljmu.ac.uk/id/eprint/28159

View Item