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Young, AJ, Pritchard, J, Lowe, GM, Buttle, L and Crampton, V (2017) Reconstitution of muscle F-actin from Atlantic salmon (Salmo salar L.) with carotenoids – binding characteristics of astaxanthin and canthaxanthin. Aquaculture Nutrition, 23 (6). pp. 1296-1303. ISSN 1365-2095
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Abstract
The binding of carotenoids to the myofibrillar protein F-actin purified from the white muscle of Atlantic salmon (Salmo salar L.) was studied using in vitro reconstitution. The binding of astaxanthin and canthaxanthin was saturable, and analysis revealed the presence of a single carotenoid-binding site. The dissociation constants (Kd) for actin prepared from 2.5 Kg FW fish were 1.04 ± 0.13 μg carotenoid mg-1 actin and 0.54 ± 0.11 μgmg-1 for astaxanthin and canthaxanthin, respectively. The saturation binding level (Bmax) for astaxanthin was 1.39 ± 0.07 μgmg-1 and 1.04 ± 0.08 μgmg-1 for canthaxanthin. These values were higher for F-actin prepared from organic and small (~0.5 Kg FW) salmon than for non-organic and larger, mature fish. The structural specificity of carotenoid binding revealed a preference for carotenoids that possess a keto group at C-4 on the end-group of the molecule, but the presence of hydroxyl groups at C-3 or C-4 reduced overall binding efficiency. The study suggests that the ability of myofibrillar proteins to bind carotenoids is not a limiting factor governing the deposition of carotenoids in the muscle of salmonids.
| Item Type: | Article |
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| Additional Information: | This is the peer reviewed version of the following article: Young AJ, Pritchard J, Lowe GM, Crampton V, Buttle L. Reconstitution of muscle F-actin from Atlantic salmon (Salmo salar L.) with carotenoids—binding characteristics of astaxanthin and canthaxanthin. Aquacult Nutr. 2017;00:1–8. https://doi.org/10.1111/anu.12504, which has been published in final form at http://dx.doi.org/10.1111/anu.12504. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Uncontrolled Keywords: | 0704 Fisheries Sciences |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RS Pharmacy and materia medica S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Natural Sciences & Psychology (closed 31 Aug 19) Pharmacy & Biomolecular Sciences |
| Publisher: | Wiley: 12 months |
| Date Deposited: | 07 Sep 2016 08:59 |
| Last Modified: | 04 Sep 2021 12:34 |
| DOI or ID number: | 10.1111/anu.12504 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/4095 |
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