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Khan, KM, Nahar, L, Mannan, A, Arfan, M, Khan, GA, Hobbs, G and Sarker, SD (2017) Evaluation of Resazurin Microtiter Plate Assay and HPLCPhotodiode Array Analysis of the Roots of Asparagus adscendens. Natural Product Research. ISSN 1478-6419
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Abstract
Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Asparagus adscendens and its solid phase extraction (SPE) fractions by using resazurin microtiter assay (REMA) against gram positive and negative bacterial registered strains and to carry out HPLC-Photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against gram-negative bacteria (MIC: 1.25-2.5 mg/mL). The use of microtiter plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.
| Item Type: | Article |
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| Additional Information: | This is an Accepted Manuscript of an article published by Taylor & Francis in Natural Product Research on 19th July 2017, available online: http://www.tandfonline.com/10.1080/14786419.2017.1353509 |
| Uncontrolled Keywords: | MD Multidisciplinary |
| Subjects: | Q Science > QD Chemistry Q Science > QK Botany R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Taylor & Francis |
| Date Deposited: | 16 Jun 2017 10:20 |
| Last Modified: | 04 Sep 2021 11:25 |
| DOI or ID number: | 10.1080/14786419.2017.1353509 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/6706 |
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