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Cobley, JN, Close, GL, Bailey, DM and Davison, GW (2017) Exercise redox biochemistry: Conceptual, methodological and technical recommendations. Redox Biology, 12. pp. 540-548. ISSN 2213-2317
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Exercise redox biochemistry Conceptual, methodological and technical recommendations.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (530kB) | Preview |
Abstract
Exercise redox biochemistry is of considerable interest owing to its translational value in health and disease. However, unaddressed conceptual, methodological and technical issues complicate attempts to unravel how exercise alters redox homeostasis in health and disease. Conceptual issues relate to misunderstandings that arise when the chemical heterogeneity of redox biology is disregarded: which often complicates attempts to use redoxactive compounds and assess redox signalling. Further, that oxidised macromolecule adduct levels reflect formation and repair is seldom considered. Methodological and technical issues relate to the use of out-dated assays and/or inappropriate sample preparation techniques that confound biochemical redox analysis. After considering each of the aforementioned issues, we outline how each issue can be resolved and provide a unifying set of recommendations. We specifically recommend that investigators: consider chemical heterogeneity, use redox-active compounds judiciously, abandon flawed assays, carefully prepare samples and assay buffers, consider repair/metabolism, use multiple biomarkers to assess oxidative damage and redox signalling.
| Item Type: | Article |
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| Uncontrolled Keywords: | Science & Technology; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; Exercise; Oxidative stress; Free radical; Antioxidants; Redox signalling; INDUCED OXIDATIVE STRESS; HUMAN SKELETAL-MUSCLE; REACTIVE OXYGEN; SUPEROXIDE-DISMUTASE; HYDROGEN-PEROXIDE; NITRIC-OXIDE; IN-VIVO; CELLULAR MECHANISMS; FREE-RADICALS; VITAMIN-E |
| Subjects: | R Medicine > RC Internal medicine > RC1200 Sports Medicine |
| Divisions: | Sport & Exercise Sciences |
| Publisher: | Elsevier |
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| Date Deposited: | 13 Nov 2017 10:43 |
| Last Modified: | 04 Sep 2021 03:37 |
| DOI or ID number: | 10.1016/j.redox.2017.03.022 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/7542 |
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