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Edwards, T, Burke, PA, Smalley, HB, Gillies, L and Hobbs, G (2014) Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea. Journal of Clinical Microbiology, 52 (6). pp. 6163-6165. ISSN 0095-1137
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Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea.pdf - Accepted Version Download (425kB) | Preview |
Publisher URL: http://dx.doi.org/10.1128/JCM.00314-14
Abstract
A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 06 Biological Sciences, 07 Agricultural And Veterinary Sciences, 11 Medical And Health Sciences |
| Subjects: | Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | American Society for Microbiology |
| Related URLs: | |
| Date Deposited: | 15 Nov 2017 11:46 |
| Last Modified: | 04 Sep 2021 11:00 |
| DOI or ID number: | 10.1128/JCM.00314-14 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/7560 |
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