Ross, RA, Leon, S, Madara, JC, Schafer, D, Fergani, C, Maguire, CA, Verstegen, AM, Brengle, E, Kong, D, Herbison, AE, Kaiser, UB, Lowell, BB and Navarro, VM (2018) PACAP neurons in the ventral premammillary nucleus regulate reproductive function in the female mouse. eLife, 7. ISSN 2050-084X

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Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP, Adcyap1) is a neuromodulator implicated in anxiety, metabolism and reproductive behavior. PACAP global knockout mice have decreased fertility and PACAP modulates LH release. However, its source and role at the hypothalamic level remain unknown. We demonstrate that PACAP-expressing neurons of the ventral premamillary nucleus of the hypothalamus (PMVPACAP) project to, and make direct contact with, kisspeptin neurons in the arcuate and AVPV/PeN nuclei and a subset of these neurons respond to PACAP exposure. Targeted deletion of PACAP from the PMV through stereotaxic virally mediated cre- injection or genetic cross to LepR-i-cre mice with Adcyap1fl/fl mice led to delayed puberty onset and impaired reproductive function in female, but not male, mice. We propose a new role for PACAP-expressing neurons in the PMV in the relay of nutritional state information to regulate GnRH release by modulating the activity of kisspeptin neurons, thereby regulating reproduction in female mice.

Item Type:	Article
Uncontrolled Keywords:	mouse; neuroscience
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions:	Natural Sciences & Psychology (closed 31 Aug 19)
Publisher:	eLife Sciences Publications Ltd
Related URLs:	https://www.ncbi.nlm.nih.gov/pubmed/29905528
Date Deposited:	26 Jun 2018 11:35
Last Modified:	04 Sep 2021 10:22
DOI or ID number:	10.7554/eLife.35960
URI:	https://researchonline.ljmu.ac.uk/id/eprint/8894

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