A Bifunctional Spin Label for Ligand Recognition on Surfaces.

Hollas, MA, Webb, SJ, Flitsch, SL and Fielding, AJ (2017) A Bifunctional Spin Label for Ligand Recognition on Surfaces. Angewandte Chemie International Edition, 56 (32). pp. 9449-9453. ISSN 1521-3773

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Abstract

In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin-labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose-binding lectin, Con A, and the biotin-binding protein avidin-peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

Item Type:	Article
Uncontrolled Keywords:	03 Chemical Sciences
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Wiley
Related URLs:	https://www.ncbi.nlm.nih.gov/pubmed/28570782
Date of acceptance:	22 May 2017
Date of first compliant Open Access:	6 September 2018
Date Deposited:	06 Sep 2018 10:33
Last Modified:	04 Sep 2021 10:08
DOI or ID number:	10.1002/anie.201703929
URI:	https://researchonline.ljmu.ac.uk/id/eprint/9174

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