Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Acridone alkaloids from the stem bark of Citrus aurantium display selective cytotoxicity against breast, liver, lung and prostate human carcinoma cells

Segun, P, Ismail, FMD, Ogbole, OO, Nahar, L, Evans, AR, Ajaiyeoba, EO and Sarker, SD (2018) Acridone alkaloids from the stem bark of Citrus aurantium display selective cytotoxicity against breast, liver, lung and prostate human carcinoma cells. Journal of Ethnopharmacology, 227. pp. 131-138. ISSN 1872-7573

[img]
Preview
Text
Acridone alkaloids from the stem bark of Citrus aurantium display selective cytotoxicity against breast, liver, lung and prostate human carcinoma cells.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (777kB) | Preview

Abstract

Ethnopharmacological relevance: Citrus aurantium L. (Rutaceae) is used, either singly or as a part of a polyherbal preparation, in Nigerian traditional medicine for the management of cancer and inflammatory diseases. Currently, there is a dearth of knowledge demonstrating its anticancer potential.
Aim of the study: This study was carried out to determine the in vitro cytotoxicity of the crude extract of the stem bark of C. aurantium, identify and isolate the bioactive constituents and to establish the cytotoxicity of such constituents.
Material and Methods The powdered bark of C. aurantium was extracted with methanol at room temperature (25-34 °C) and the crude extract was partitioned successively, with nhexane, dichloromethane (DCM) and methanol. Amongst the fractions, the DCM fraction was the most active and compounds were isolated from this fraction using several chromatographic techniques. The structures of the isolated compounds were elucidated by spectroscopic means (mass spectrometry, one-dimensional and two-dimensional nuclear magnetic resonance). The cytotoxicity of the extract, and the isolated compounds were evaluated by the MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium) assay against four human cancer cell lines: A549 (lung), HepG2 (liver), MCF7 (breast) and PC3 (prostate). The selectivity of the isolated compounds was assessed using the normal human prostate epithelium cells (PNT2).
Results and Discussion: Of the three plant fractions, the DCM fraction showed significant cytotoxicity, with its highest activity against A549 cells (IC50 = 3.88 µg/mL) and the least activity on HepG2 cells (IC50 = 5.73 µg/mL). Six acridone alkaloids: citrusinine-I (1), citracridone-I (2), 5-hydroxynoracronycine (3), natsucitrine-I (4), glycofolinine (5) and citracridone-III (6), were isolated from the DCM fraction of C. aurantium. The isolated compounds demonstrated potent to moderate cytotoxicity ((IC50 = 12.65 – 50.74 µM) against the cancer cells under investigation. It is noteworthy that the compounds exerted cytotoxicity at least four times more selective towards the carcinoma cells than the PNT2 cells.
Conclusion: The results obtained from this study have provided some evidence for the ethnomedicinal use of C. aurantium against cancer and the acridone alkaloids present in its stem bark, have appeared to be responsible for this effect. Further research to explore the underlying molecular mechanism of the isolated acridone alkaloids is needful.

Item Type: Article
Uncontrolled Keywords: 0607 Plant Biology, 1115 Pharmacology And Pharmaceutical Sciences, 1104 Complementary And Alternative Medicine
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Date Deposited: 12 Sep 2018 09:28
Last Modified: 04 Sep 2021 10:08
DOI or ID number: 10.1016/j.jep.2018.08.039
URI: https://researchonline.ljmu.ac.uk/id/eprint/9192
View Item View Item