Tools
Tools
Kist, AM, Lampert, A and O'Reilly, AO (2018) DIsulfide Mapping PLanner Software Tool. Journal of Computaional Biology, 25 (4). ISSN 1066-5277
|
Text
DIsulfide Mapping PLanner Software Tool.pdf - Accepted Version Download (364kB) | Preview |
Abstract
Disulfide bridges are side-chain-mediated covalent bonds between cysteines that stabilize many protein structures. Disulfide mapping experiments to resolve these linkages typically involve proteolytic cleavage of the protein of interest followed by mass spectroscopy to identify fragments corresponding to linked peptides. Here we report the sequence-based “DIMPL” web tool to facilitate the planning and analysis steps of experimental mapping studies. The software tests permutations of user-selected proteases to determine an optimal peptic digest that produces cleavage between cysteine residues, thus separating each to an individual peptide fragment. The webserver returns fragment sequence and mass data that can be dynamically ordered to enable straightforward comparative analysis with mass spectroscopy results, facilitating dipeptide identification.
| Item Type: | Article |
|---|---|
| Additional Information: | Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/cmb.2017.0129 |
| Uncontrolled Keywords: | 01 Mathematical Sciences, 06 Biological Sciences, 08 Information And Computing Sciences |
| Subjects: | Q Science > QA Mathematics > QA76 Computer software Q Science > QD Chemistry |
| Divisions: | Natural Sciences & Psychology (closed 31 Aug 19) |
| Publisher: | Mary Ann Liebert |
| Related URLs: | |
| Date Deposited: | 04 Oct 2018 10:33 |
| Last Modified: | 04 Sep 2021 02:22 |
| DOI or ID number: | 10.1089/cmb.2017.0129 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/9416 |
 |
View Item |