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NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice

Fergani, C, Leon, S, Padilla, SL, Verstegen, AMJ, Palmiter, RD and Navarro, VM (2018) NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice. eLife, 7. ISSN 2050-084X

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Abstract

Neurokinin B (NKB) signaling is critical for reproduction in all studied species. The existing consensus is that NKB induces GnRH release via kisspeptin (Kiss1) stimulation in the arcuate nucleus. However, the stimulatory action of NKB is dependent on circulating estrogen (E2) levels, without which, NKB inhibits luteinizing hormone (LH) release. Importantly, the evidence supporting the kisspeptin-dependent role of NKB, derives from models of persistent hypogonadal state [e.g. Kiss1r knock-out (KO) mice], with reduced E2 levels. Here, we demonstrate that in the presence of E2, NKB signaling induces LH release in a kisspeptin-independent manner through the activation of NK3R (NKB receptor) neurons in the posterodorsal medial amygdala (MePD). Importantly, we show that chemogenetic activation of MePD Kiss1 neurons induces LH release, however, the stimulatory action of NKB in this area is Kiss1 neuron-independent. These results document the existence of two independent neuronal circuitries within the MePD that regulate reproductive function in females.

Item Type: Article
Uncontrolled Keywords: Science & Technology; Life Sciences & Biomedicine; Biology; Life Sciences & Biomedicine - Other Topics; LUTEINIZING-HORMONE SECRETION; NEUROKININ-B; ARCUATE NUCLEUS; KISS1 NEURONS; RECEPTORS; RAT; PROJECTIONS; ACTIVATION; JUVENILE; SYSTEM
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Natural Sciences and Psychology
Publisher: eLife Sciences Publications Ltd
Related URLs:
Date Deposited: 29 Jan 2019 12:19
Last Modified: 29 Jan 2019 12:23
DOI or Identification number: 10.7554/eLife.40476
URI: http://researchonline.ljmu.ac.uk/id/eprint/10061

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