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The serum opsonin L-ficolin is detected in lungs of human transplant recipients following fungal infections and modulates inflammation and killing of Aspergillus fumigatus.

Bidula, S, Sexton, DW, Abdolrasouli, A, Shah, A, Reed, A, Armstrong-James, D and Schelenz, S (2015) The serum opsonin L-ficolin is detected in lungs of human transplant recipients following fungal infections and modulates inflammation and killing of Aspergillus fumigatus. Journal of Infectious Diseases, 212 (2). pp. 234-246. ISSN 1537-6613

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L-ficolin, is detected in human lungs of transplant patients following fungal infections and modulates inflammation and killing of A. fumigatus 2015 accepted version (1).pdf - Accepted Version

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Abstract

BACKGROUND: Invasive aspergillosis (IA) is a life-threatening systemic fungal infection in immunocompromised individuals that is caused by Aspergillus fumigatus. The human serum opsonin, L-ficolin, has been observed to recognize A. fumigatus and could participate in fungal defense. METHODS: Using lung epithelial cells, primary human monocyte-derived macrophages (MDMs), and neutrophils from healthy donors, we assessed phagocytosis and killing of L-ficolin-opsonized live A. fumigatus conidia by flow cytometry and microscopy. Additionally, cytokines were measured by cytometric bead array, and L-ficolin was measured in bronchoalveolar lavage (BAL) fluid from lung transplant recipients by enzyme-linked immunosorbent assay. RESULTS: L-ficolin opsonization increased conidial uptake and enhanced killing of A. fumigatus by MDMs and neutrophils. Opsonization was also shown to manifest an increase in interleukin 8 release from A549 lung epithelial cells but decreased interleukin 1β, interleukin 6, interleukin 8, interleukin 10, and tumor necrosis factor α release from MDMs and neutrophils 24 hours after infection. The concentration of L-ficolin in BAL fluid from patients with fungal infection was significantly higher than that for control subjects (P = .00087), and receiving operating characteristic curve analysis highlighted the diagnostic potential of L-ficolin for lung infection (area under the curve, 0.842; P < .0001). CONCLUSIONS: L-ficolin modulates the immune response to A. fumigatus. Additionally, for the first time, L-ficolin has been demonstrated to be present in human lungs.

Item Type: Article
Additional Information: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record J Infect Dis. (2015) 212 (2): 234-246. is available online at: http://dx.doi.org/10.1093/infdis/jiv027
Uncontrolled Keywords: 11 Medical And Health Sciences, 06 Biological Sciences
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Oxford University Press
Related URLs:
Date Deposited: 14 Sep 2015 14:35
Last Modified: 22 Jan 2016 00:50
DOI or Identification number: 10.1093/infdis/jiv027
URI: http://researchonline.ljmu.ac.uk/id/eprint/1994

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