Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Evaluation of Resazurin Microtiter Plate Assay and HPLCPhotodiode Array Analysis of the Roots of Asparagus adscendens

Khan, KM and Nahar, L and Mannan, A and Arfan, M and Khan, GA and Hobbs, G and Sarker, SD (2017) Evaluation of Resazurin Microtiter Plate Assay and HPLCPhotodiode Array Analysis of the Roots of Asparagus adscendens. Natural Product Research. ISSN 1478-6419

[img] Text
NPR_2 REVISION.pdf - Accepted Version
Restricted to Repository staff only until 19 July 2018.

Download (1MB)

Abstract

Asparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Asparagus adscendens and its solid phase extraction (SPE) fractions by using resazurin microtiter assay (REMA) against gram positive and negative bacterial registered strains and to carry out HPLC-Photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against gram-negative bacteria (MIC: 1.25-2.5 mg/mL). The use of microtiter plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.

Item Type: Article
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Natural Product Research on 19th July 2017, available online: http://www.tandfonline.com/10.1080/14786419.2017.1353509
Uncontrolled Keywords: MD Multidisciplinary
Subjects: Q Science > QD Chemistry
Q Science > QK Botany
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Taylor & Francis
Date Deposited: 16 Jun 2017 10:20
Last Modified: 14 Sep 2017 06:47
DOI or Identification number: 10.1080/14786419.2017.1353509
URI: http://researchonline.ljmu.ac.uk/id/eprint/6706

Actions (login required)

View Item View Item