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Comparative study of the production of rhamnolipid biosurfactants by B. thailandensis E264 and P. aeruginosa ATCC 9027 using foam fractionation

Diaz De Rienzo, MA and Kamalanathan, ID and Martin, PJ (2016) Comparative study of the production of rhamnolipid biosurfactants by B. thailandensis E264 and P. aeruginosa ATCC 9027 using foam fractionation. Process Biochemistry, 51 (7). pp. 820-827. ISSN 1359-5113

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Abstract

Biosurfactants are surface-active agents that are produced by a variety of microorganisms including yeasts, filamentous fungi and bacteria. In this work, we report on the ability of Pseudomonas aeruginosa ATCC 9027 and Burkholderia thailandensis E264 to produce rhamnolipids via a 10-L bioreactor and their recovery through foam fractionation studies in a continuous stripping mode. The recovery of Rha-C10-C10 (mono-rhamnolipids) produced by P. aeruginosa ATCC 9027 increased (from 6% to 96%), whilst the enrichment decreased (from 2.9 to 1.2) with the increasing airflow rate. These results are consistent with foam fractionation of a single surfactant system with stable foam. The recovery and enrichment of Rha-Rha-C14-C14 (di-rhamnolipids) produced by B. thailandensis E264 (and an unknown molecule) in a single-component system were found to display different characteristics. Both recovery and enrichment were found to decrease with the airflow rate. It is postulated that a competitive adsorption process could occur between the smaller molecule identified by electrospray ionisation–mass spectrometry (ESI–MS) and Rha-Rha-C14-C14.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry And Cell Biology
Subjects: Q Science > QR Microbiology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Date Deposited: 28 Sep 2017 11:05
Last Modified: 28 Sep 2017 11:05
DOI or Identification number: 10.1016/j.procbio.2016.04.007
URI: http://researchonline.ljmu.ac.uk/id/eprint/7234

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