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Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium

Edwards, T and Burke, P and Smalley, HB and Gillies, L and Longhurst, D and Vipond, B and Hobbs, G (2015) Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium. Diagnostic Microbiology and Infectious Disease, 83 (1). pp. 13-17. ISSN 0732-8893

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Abstract

Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner. A LAMP assay was designed to the pdhD gene of M. genitalium, and the limit of detection of the assay was determined as 10. fg of M. genitalium genomic DNA, equating to ~16 copies of the M. genitalium genome, which was equally sensitive as a gold standard 16S rRNA polymerase chain reaction assay. © 2015 Elsevier Inc.

Item Type: Article
Uncontrolled Keywords: 0605 Microbiology, 1103 Clinical Sciences, 1108 Medical Microbiology
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Date Deposited: 15 Nov 2017 11:16
Last Modified: 15 Nov 2017 11:16
DOI or Identification number: 10.1016/j.diagmicrobio.2015.05.010
URI: http://researchonline.ljmu.ac.uk/id/eprint/7558

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