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Reliability of Protein Abundance and Synthesis Measurements in Human Skeletal Muscle.

Srisawat, K, Hesketh, KL, Cocks, MS, Strauss, JA, Edwards, BJ, Lisboa, PJ, Shepherd, SO and Burniston, JG (2019) Reliability of Protein Abundance and Synthesis Measurements in Human Skeletal Muscle. Proteomics. ISSN 1615-9853

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Abstract

We investigated the repeatability of dynamic proteome profiling (DPP), which is a novel technique for measuring the relative abundance (ABD) and fractional synthesis rate (FSR) of proteins in humans. LC-MS analysis was performed on muscle samples taken from male participants (n = 4) that consumed 4 × 50 ml doses of deuterium oxide (2 H2 O) per day for 14 d. ABD was measured by label-free quantitation and FSR was calculated from time-dependent changes in peptide mass isotopomer abundances. One-hundred and one proteins had at least 1 unique peptide and were used in the assessment of protein ABD. Fifty-four of these proteins met more stringent criteria and were used in the assessment of FSR data. The median (M), lower- (Q1 ) and upper-quartile (Q3 ) values for protein FSR (%/d) were M = 1.63, Q1  = 1.07, Q3  = 3.24. The technical CV of ABD data had a median value of 3.6% (Q1 1.7% - Q3 6.7%), whereas the median CV of FSR data was 10.1% (Q1 3.5% - Q3 16.5%). These values compare favorably against other assessments of technical repeatability of proteomics data, which often set a CV of 20% as the upper bound of acceptability. This article is protected by copyright. All rights reserved.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Reliability of Protein Abundance and Synthesis Measurements in Human Skeletal Muscle, which has been published in final form at 10.1002/pmic.201900194. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Uncontrolled Keywords: 06 Biological Sciences, 08 Information and Computing Sciences, 11 Medical and Health Sciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QM Human anatomy
Q Science > QP Physiology
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sports & Exercise Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 04 Dec 2019 10:04
Last Modified: 20 Dec 2019 08:45
DOI or Identification number: 10.1002/pmic.201900194
URI: http://researchonline.ljmu.ac.uk/id/eprint/11839

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