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Adaptation of rat fast-twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis.

Hesketh, SJ, Sutherland, H, Lisboa, PJ, Jarvis, JC and Burniston, JG (2020) Adaptation of rat fast-twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis. FASEB Journal. ISSN 1530-6860

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Abstract

Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide (2 H2 O) labeling and chronic low-frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance, and degradation of individual proteins during exercise-induced muscle adaptation. Independent groups of rats received CLFS (10 Hz, 24 h/d) and 2 H2 O for 0, 10, 20, or 30 days. The extensor digitorum longus (EDL) was isolated from stimulated (Stim) and contralateral non-stimulated (Ctrl) legs. Proteomic analysis encompassed 38 myofibrillar and 46 soluble proteins and the rates of change in abundance, synthesis, and degradation were reported in absolute (ng/d) units. Overall, synthesis and degradation made equal contributions to the adaptation of the proteome, including instances where a decrease in protein-specific degradation primarily accounted for the increase in abundance of the protein.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry and Cell Biology, 0606 Physiology, 1116 Medical Physiology
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Applied Mathematics
Sports & Exercise Sciences
Publisher: Federation of American Society of Experimental Biology
Related URLs:
Date Deposited: 09 Jul 2020 09:58
Last Modified: 09 Jul 2020 10:00
DOI or Identification number: 10.1096/fj.202000668RR
URI: http://researchonline.ljmu.ac.uk/id/eprint/13275

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