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Olakanye, AO and Ralebitso-Senior, TK (2018) Soil metabarcoding identifies season indicators and differentiators of pig and Agrostis/Festuca spp decomposition. Forensic Science International, 288. pp. 53-58. ISSN 0379-0738
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Olakanye and Ralebitso-Senior_Soil metabarcoding and decomposition_FSI_2018.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (707kB) | Preview |
Abstract
To gain a better understanding of how environmental microbiota respond to cadaver decomposition, a forensic ecogenomic study was made with soil only control and 4 g each of S. scrofa domesticus and plant litter (Agrostis/Festuca spp) buried individually in a sandy clay loam (80 g) in sealed but perforated triplicate microcosms. The next-generation sequencing (Illumina Miseq) of the soil bacteria (16S rRNA gene) clade revealed seasonal taxomonic shifts at genus-level for the pig and plant litter microcosms compared to the non-burial controls. In particular, numerical abundances of Sphingobacterium (5.9%) and Pedobacter (24.1%) for the pig microcosms, and Rhodanobacter (18.1%) and Shinella (4.6%) for the plant litter microcosms, identified bacterial genera that could be tracked to establish a (seasonal) subsurface postmortem microbial clock. Also, family-level resolution revealed members that were unique to the control, grass and pig soils after 365 days.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MD Multidisciplinary |
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Elsevier |
| Date Deposited: | 10 Apr 2018 10:55 |
| Last Modified: | 16 Jan 2023 16:17 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/8220 |
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