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Boesenbergia rotunda (L.) Mansf. extract potentiate the antibacterial activity of some β-lactams against β-lactam-resistant Staphylococci.

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Teethaisong, Y, Pimchan, T, Srisawat, R, Hobbs, G and Eumkeb, G (2017) Boesenbergia rotunda (L.) Mansf. extract potentiate the antibacterial activity of some β-lactams against β-lactam-resistant Staphylococci. Journal of Global Antimicrobial Resistance. ISSN 2213-7173

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Abstract

OBJECTIVE: The purpose of this study was to investigate the effect of Boesenbergia rotunda (L.) Mansf. Extract (BRE) and peptidoglycan inhibitor antibiotics, either used alone or in combination against β-lactam resistant Staphylococci. METHODS: Antibacterial and synergistic activities of BRE alone and in combination with ampicillin (AMP), cloxacillin (CLX), cefazolin (CFZ) or vancomycin (VAN) were evaluated against two β-lactam-resistant Staphylococcus aureus (BRSA) and one β-lactam-resistant Staphylococcus epidermidis (BRSE). The activities were confirmed by killing curve assays. The preliminary antimicrobial action was elucidated by transmission electron microscopy (TEM) and cytoplasmic membrane (CM) permeability. RESULTS: All tested Staphylococci were inhibited by BRE at a MIC of 16μg/mL. Two BRSA strains showed high resistance to CLX, AMP, and CFZ, while BRSE was resistant to CLX and AMP. All tested isolates remained susceptible to VAN. The chequerboard assay demonstrated the fractional inhibitory concentration index (FICI) at 0.502 of the BRE and CLX combination against the BRSA strains. Killing curve determinations confirmed the antibacterial and synergistic activities. The TEM study revealed collapse of the CM in BRE-treated cells and damage of both CM and peptidoglycan in BRE plus CLX-treated cells. The CM permeability assay showed that either BRE or nisin alone, and BRE plus CLX significantly induced leakage of OD260-absorbing materials. CONCLUSIONS: The BRE potentiated β-lactams, particularly CLX against β-lactam-resistant Staphylococci by damaging the CM and peptidoglycan layer, leading to leakage of intracellular materials. The combination of BRE and β-lactams provides a potential way forward in developing a novel antistaphylococcal agent.

Item Type: Article
Uncontrolled Keywords: β -lactams; Boesenbergia rotunda (L.) Mansf. extract; Staphylococci; cytoplasmic membrane
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
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Date Deposited: 02 Feb 2018 11:37
Last Modified: 03 Sep 2021 23:34
DOI or ID number: 10.1016/j.jgar.2017.10.019
URI: https://researchonline.ljmu.ac.uk/id/eprint/7924
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